Plaque Assay - Determining viral concentration of a sample
Definition: Assay for virus in which a dilute solution of the virus is applied to a culture dish containing a layer of the host cells, convective spread is prevented by making the medium very viscous. After incubation the plaques, areas in which cells have been killed (or transformed), can be recognised and the number of infective virus particles in the original suspension estimated.
Let us recap the process of performing a plaque assay with the following clip.
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